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tRNA Probes

Transcription

SP6  RNA  Polymerase

Item

Description

Quantity per tube

Cost per tube

SP6
SP6 RNA Polymerase    50 μl    $ 50

 

Transcription  Components

TCM contains 6 mM ATP, CTP. GTP, and UTP in 160 mM HEPES (pH 7.5), 32 mM MgCl2, 4 mM spermidine, 20 mM DTT, 1 u/μl RNasin, and 0.01 u/μl pyrophosphatase.  TCM+GG contains  diguanosine triphosphate (GpppG, GE #27-4743-01) and the GTP concentration is 0.6 mM.

 

Item

Description

Quantity per tube

Cost per tube

TCM
2x Transcription Mix (without GpppG) 50 μl   $ 15
TCM+GG 2x Transcription Mix (with GpppG) 50 μl  $ 45