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tRNA Probes

E. coli S-100 Soluble Cytosolic Proteins

Following MRE 600 E. coli cell disruption, DNase treatment, and sedimentations to remove membranes and ribosomes, the 100,000g supernatant is chromatographed over Sephadex G-25 to remove small molecules, then DEAE-cellulose to remove tRNA and other nucleic acids, and finally Sephadex G-25 again to remove salt left in the sample by the ion exchange chromatography. Included among the S-100 proteins are all of the aminoacyl-tRNA synthetases and the initiation, elongation, and termination factors required for translation.

 

Item

Description

Quantity per tube

Cost per tube

S-100
S-100 Soluble Cytosolic Proteins  0.5 ml   $ 30