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tRNA Probes

Acetylated aa–tRNAs

Acetylated Lys-tRNALys/amb (Nε-acetyl-Lys-tRNA or εAc-Lys-tRNA) incorporates an uncharged amino acid (εAc-Lys) into a nascent protein instead of a charged Lys during in vitro translation.  εAc-Lys-tRNALys/amb is an appropriate control for samples containing ε-modified Lys-tRNALys/amb with a photo-reactive, fluorescent, or other probe.  Each tube contains 400 pmoles of aa-tRNA that have been dialyzed into 25-30 μl of 1 mM KOAc (pH 5.0), 2 mM Mg(OAc)2.

Item #

Acetylated  aa–tRNAs

Quantity per tube

Cost per tube

A-11

εAc-[14C]Lys-tRNAamb

400 pmoles

$ 60
A-21

εAc-[3H]Lys-tRNAamb

400 pmoles

$ 60
A-51

non-RA εAc-Lys-tRNAamb

400 pmoles $ 60
 
L-11

E. coli  εAc-[14C]Lys-tRNALys

400 pmoles

$ 55
L-21

E. coli  εAc-[3H]Lys-tRNALys

400 pmoles

$ 55
L-51

non-RA E. coli  εAc-Lys-tRNALys

400 pmoles $ 55
 
L-31

yeast  εAc-[14C]Lys-tRNALys

400 pmoles

$ 60
L-41

yeast  εAc-[3H]Lys-tRNALys

400 pmoles

$ 60
L-61

non-RA yeast  εAc-Lys-tRNALys

400 pmoles $ 60